This past week, Dr. Kristina Cammen and undergraduate research assistant, Liz Piotrowski, collected their first eDNA water samples at the Marine Mammals of Maine (MMoME) triage facility located in Harpswell, Maine. Thank you to Dominique Walk, MMoME’s assistant stranding coordinator, and several MMoME interns for their collaboration in this effort.
eDNA, short for environmental DNA, is free floating DNA that is left behind by an organism as it travels through an environment. We are working to develop protocols that will allow us to isolate and characterize this DNA, for example from a water sample collected in a seal rehab pool and ultimately from seawater samples collected near seal haul-out sites. Our hope is that these non-invasive sampling methods will enable future investigations of the genetic diversity and population structure of seals in the Gulf of Maine.
9/29/18: Updated to add that our first attempts to detect and sequence seal DNA from the MMoME facility have been successful! We are now working to optimize our protocols so that we can distinguish between individuals that are present in the same pool of water.